cycloheximide

[ sahy-kloh-hek-suh-mahyd, -mid ]

noun

, Pharmacology.

1. an antibiotic substance, C 15 H 23 NO 4 , isolated from the bacterium Streptomyces griseus, used experimentally to block protein synthesis.

Word History and Origins

Origin of cycloheximide¹

First recorded in 1945–50; cyclohex(ane) + imide

Example Sentences

The ∆VIM mutant is deleted for amino acids 622–625. b, Serial tenfold dilutions of wild-type and vms1Δ cells transformed with the indicated vms1 alleles were spotted on YPD plates containing 10 or 25 ng ml−1 cycloheximide and incubated at 30 °C for 3 days.

From Nature

Hadjiolova, K. V., Nicoloso, M., Mazan, S., Hadjiolov, A. A. & Bachellerie, J. P. Alternative pre-rRNA processing pathways in human cells and their alteration by cycloheximide inhibition of protein synthesis.

From Nature

The cells were then either transfected with an empty p3×-Flag vector or Flag-tagged AIMP2 vector and grown for another 48 h before the addition of cycloheximide.

From Nature

Immediately before sample collection, cultured mammalian cells were incubated with media containing 100 µgml cycloheximide for 10min at 37°C to stop translation elongation.

From Nature

Soon after fertilization the jelly membranes of X. laevis embryos were chemically removed, and at the desired stages, embryos were flash frozen in lysis buffer without cycloheximide pre-treatment.

From Nature